High-Quality Drug Test Panel Factory: AMP/BAR/BZO/COC/MOP/THC

The one step AMP/BAR/BZO/COC/MOP/THC Test Panel is a lateral flow chromatographic immunoassay for the detection of Amphetamine, Barbiturates, Cocaine, Methamphetamine, Opiate and Marijuana in human urine.1.Baselt, R.C. Disposition of Toxic Drugs and Chemicals in Man, Biomedical Publications, 1982. 2.Urine Testing for Drugs of Abuse. National Institute on Drug Abuse (NIDA), Research Monograph 73, 1986. 3.Thomas L. eds., Labor und Diagnose, 6. ed., TH-Books Verlags gesellschaft, Frankfurt, 2005 4.Fed. Register, Department of Health and Human Services, Mandatory Guidelines for Federal Workplace Drug Testing Programs, 53, 69, 11970, 1988. 5.McBay, A.J. Clin. Chem. 33, 33B-40B, 1987.✽ The kit should be stored at 2-30°C until the expiry date printed on the sealed pouch. ✽ The test must remain in the sealed pouch until use. ✽ Do not freeze. ✽ Cares should be taken to protect components in this kit from contamination. Do not use if there is evidence of microbial contamination or precipitation. Biological contamination of dispensing equipments, containers or reagents can lead to false results.✔ The AMP/BAR/BZO/COC/MOP/THC Test Panel is intended only for use with human urine specimens. ✔ Collected urine specimens must be put in clear and dry containers. Ensure that a sufficient quantity of the specimen is collected to allow submerging the dipping area of the strip. ✔ Perform the testing immediately after the specimen collection. Do not leave the specimens at room temperature for prolonged periods. Specimens may be stored at 2-8°C for up to 48 hours. For long term storage, specimens should be kept below -20°C. ✔ Bring specimens to room temperature prior to testing. Frozen specimens must be completely thawed and mixed well prior to testing. Avoid repeated freezing and thawing of specimens. ✔ Pack the specimens in compliance with applicable regulations for transportation of etiological agents, in case they need to be shipped.Bring tests, specimens, and/or controls to room temperature (15-30°C) before use. 1.Remove the panel from its sealed pouch and use it as soon as possible. To obtain a best result, the assay should be performed within one hour. 2.Collect the urine in a urine cup, insert the test panel into the urine and make sure the urine do not pass the max line. 3.The result should be read at 5 minutes. Do not interpret the result after 20 minutes.

POSITIVE RESULT:	* A colored band appears in the control band region (C) and another colored band appears in the T band region.
NEGATIVE RESULT:	One colored band appears in the control band region (C). No band appears in the test band region (T).
INVALID RESULT:	Control band fails to appear. Results from any test which has not produced a control band at the specified reading time must be discarded. Please review the procedure and repeat with a new test. If the problem persists, discontinue using the kit immediately and contact your local distributor.

1.The AMP/BAR/BZO/COC/MOP/THC Test Panel is for professional in vitro diagnostic use, and should be used for the qualitative detection of morphine and other opiates only. 2.The AMP/BAR/BZO/COC/MOP/THC Test Panel provides only a quantitative, preliminary analytical result. A secondary analytical method must be used to obtain a confirmed result. Gas chromatography/mass spectrophotometry (GC/MS) is the preferred confirmatory method. 3.Please take the specificity and the cross reactivity into account for evaluation 4.A positive result with any of the tests indicates the presence of a drug/metabolite only, and does not indicate or measure intoxication. 5.A negative result may not necessarily indicate drug-free urine. Negative results can be obtained when drug is present but below the cut-off level of the test. 6.There is a possibility that technical or procedural errors as well as other substances and factors not listed may interfere with the test and cause false results. 7.The test is designed for use with human urine only. Due to absence of ions and other components in pure water the usage or pure water for test could lead to false or invalid results. 8.Foods and tea containing poppy products (the origin of opiates) may also produce a positive result. 9.The test does not distinguish between drugs of abuse and certain medications.A drug-free urine pool was spiked with drugs at the following concentrations: +/- 25%, +/- 50% and cutoff. The data are summarized below:AMP/BAR/BZO/COC/MOP/THC Test Panel has been designed to detect Amphetamine, Barbiturates, Benzodiazepines, Cocaine, Morphine and Marijuana through visual interpretation of color development in the strip. The membrane was immobilized with Drug antigens conjugates on the test region, and the sample pad was pre-coated with colored anti- drugs antibodies colloidal gold conjugates. After specimens were added, the gold-conjugates move along the membrane chromatographically by capillary action and antibodies get to the test region. If there is no drug molecule in the urine the antibody gold conjugate would attach to the drug conjugate to form a visible line. Therefore, the formation of a visible precipitant in the test region occurs when the urine is negative for the drug. If Amphetamine, Barbiturates, Cocaine, Methamphetamine, Opiate and Marijuana are present in the urine, the drug antigen competes with the immobilized drug conjugate on the test region for limited antibody sites. In case of sufficient concentration of the drug, it fills the limited antibody binding sites. This will prevent attachment of the colored antibody-colloidal gold conjugate to the drug conjugate zone on the test region. Therefore, absence of the colored band on the test region indicates a positive result. Appearance of a colored band at the control region serves as a procedural control. This indicates that proper volume of specimen has been added and membrane wicking has occurred.

Test Panels

Disposable pipettes

Packaging Insert

1.The intensity of the color in test region (T) may vary depending on the concentration of aimed substances present in the specimen. Therefore, any shade of color in the test region should be considered negative. Besides, the concentration level can not be determined by this qualitative test. 2.Insufficient specimen volume, incorrect operation procedure, or performing expired tests are the most likely reasons for control band failure.★ Internal procedural controls are included in the test. A colored band appearing in the control region (C) is considered an internal positive procedural control. It confirms sufficient specimen volume and correct procedural technique. ★ External controls are not supplied with this kit. It is recommended that positive and negative controls be tested as a good laboratory practice to confirm the test procedure and to verify proper test performance

Test	Calibrator	Cutoff
AMP	Amphetamine	1000ng/ml
BAR	Barbiturates	300ng/ml
BZO	Benzodiazepines	300ng/ml
COC	Cocaine	300ng/ml
MOP	Morphine (Opiate)	300ng/ml
THC	Marijuana	50ng/ml

This assay provides only a preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.★ For professional in vitro diagnostic use only. ★ Do not use after expiration date indicated on the package. Do not use the test if its foil pouch is damaged. Do not reuse tests. ★ This kit contains products of animal origin. Certified knowledge of the origin and/or sanitary state of the animals does not totally guarantee the absence of transmissible pathogenic agents. It is therefore, recommended that these products be treated as potentially infectious, and handled observing the usual safety precautions (do not ingest or inhale). ★ Avoid cross-contamination of specimens by using a new specimen collection container for each specimen obtained. ★ Read the entire procedure carefully prior to performing any tests. ★ Do not eat, drink or smoke in the area where the specimens and kits are handled. Handle all specimens as if they contain infectious agents. Observe established precautions against microbiological hazards throughout the procedure and follow the standard procedures for proper disposal of specimens. Wear protective clothing such as laboratory coats, disposable gloves and eye protection when specimens are assayed. ★ Humidity and temperature can adversely affect results. ★ The used testing materials should be discarded in accordance with local, state and/or federal regulations.

Timer

Specimen collection container

Percent of Cut-off	n	AMP		BAR		BZO
Percent of Cut-off	n	-	+	-	+	-	+
0	30	30	0	30	0	30	0
-50%	30	30	0	30	0	30	0
-25%	30	23	7	28	2	30	0
Cut-off	30	9	21	4	26	3	27
+25%	30	1	29	0	30	0	30
+50%	30	0	30	0	30	0	30

Percent of Cut-off	n	COC		MOP		THC
Percent of Cut-off	n	-	+	-	+	-	+
0	30	30	0	30	0	30	0
-50%	30	30	0	30	0	30	0
-25%	30	30	0	27	3	30	0
Cut-off	30	4	26	5	25	7	23
+25%	30	0	30	0	30	0	30
+50%	30	0	30	0	30	0	30

The following table lists compounds that are positively detected in urine by the AMP One Step Amphetamine Test Strip (Urine) at 5 minutes.

Related Compound	Concentration (ng/mL)
AMP
D-Amphetamine	1,000
D,L-Amphetamine sulfate	3,000
L-Amphetamine	50,000
(±) 3,4-MethylenedioxyAmphetamine	2,000
Phentermine	3,000
BAR
Secobarbital	300
Amobarbital	300
Alphenol	150
Aprobarbital	200
Butalbital	2,500
Butethal	100
Cyclopentobarbital	600
Pentobarbital	300
Butabarbital	75
Phenobarbital	100
BZO
Alprazolam	125
Bromazepam	625
Chlordiazepoxide	2,500
Clobazam	63
Clonazepam	2,500
Clorazepate dipotassium	3,330
Delorazepam	2,500
Desalkylflurazepam	250
Diazepam	250
Estazolam	5,000
Flunitrazepam	375
(±) Lorazepam	1,250
RS-Lorazepam glucuronide	1,250
Midazolam	100,000
Nitrazepam	25,000
Norchlordiazepoxide	250
Nordiazepam	500
Oxazepam	300
Sulindac	100,000
Temazepam	63
Triazolam	5,000
COC
Benzoylecgonine	300
Cocaine HCl	780
Cocaethylene	12,500
MOP
Codeine	300
Ethylmorphine	6,250
Hydrocodone	50,000
Hydromorphone	3,125
Levophanol	1,500
6-Monoacethylmorphine	400
Morphine	300
Morphine 3-b-D-glucuronide	1,000
Norcodeine	6,250
Normorphone	100,000
Oxycodone	30,000
Oxymorphone	100,000
Procaine	15,000
Thebaine	6,250
THC
11-nor-Δ9-THC-9-COOH	50
11-nor-Δ8-THC-9-COOH	50
11-hydroxy-Δ9-Tetrahydrocannabinol	20000
Δ8-Tetrahydrocannabinol	15000
Δ9-Tetrahydrocannabinol	20000
Cannabinol	20000
Cannabidiol	100000

4-Acetamidophenol	Estrone-3-sulfate	Oxolinic acid
Acetophenetidin	Ethyl-p-aminobenzoate	Oxycodone
N-Acetylprocainamide	Fenfluramine	Oxymetazoline
Acetylsalicylic acid	Fenoprofen	Papaverine
Aminopyrine	Furosemide	Penicillin-G
Amitryptyline	Gentisic acid	Pentazocine
Amobarbital	Hemoglobin	Pentobarbital
Amoxicillin	Hydralazine	Perphenazine
Ampicillin	Hydrochlorothiazide	Phencyclidine
L-Ascorbic acid	Hydrocodone	Phenelzine
Apomorphine	Hydrocortisone	Phenobarbital
Aspartame	p-HydroxyAmphetamine	L-Phenylephrine
Atropine	O-Hydroxyhippuric acid	Brompheniramine
Benzilic acid	3-Acetate	Phenylpropanolamine
Benzoic acid	Acetaminophen	Prednisolone
Benzoylecgonine	3-Hydroxytyramine	Prednisone
Benzphetamine	Ibuprofen	Procaine
Bilirubin	Imipramine	Promazine
(±) - Brompheniramine	(±) - Isoproterenol	Promethazine
Caffeine	Isoxsuprine	D,L-Propanolol
Cannabidiol	Ketamine	D-Propoxyphene
Cannabinol	Ketoprofen	D-Pseudoephedrine
Chloralhydrate	Labetalol	Quinidine
Chloramphenicol	Levorphanol	Quinine
Chlordiazepoxide	Loperamide	Ranitidine
Chlorothiazide	Maprotiline	Salicylic acid
(±) Chlorpheniramine	Meperidine	Secobarbital
Chlorpromazine	Meprobamate	Nalorphine
Chlorquine	Methadone	Iproniazid
Cholesterol	D-methAmphetamine	Sulfamethazine
Clomipramine	(L)-methAmphetamine	Sulindac
Clonidine	Methoxyphenamine	Temazepam
Cocaine hydrochloride	Tetrahydrocortisone,	Tetracycline
Codeine	Phentermine	Acetone
Cortisone	L-Epinephrine	(-)-ψ-Ephedrine
(-) Cotinine	b-Phenylethylamine	(-) Y Ephedrine
Creatinine	Methylphenidate	Chlorpheniramin
Deoxycorticosterone	(1R,2S)-(-)-Ephedrine	Tetrahydrozoline
Dextromethorphan	D,L-Amphetamine sulfate	Thebaine
Diazepam	Nalidixic acid	Thiamine
Diclofenac	Naloxone	Thioridazine
Diflunisal	Naltrexone	Tolbutamine
Digoxin	Naproxen	Triamterene
Diphenhydramine	Niacinamide	Trifluoperazine
Doxylamine	Nifedipine	Trimethoprim
Ecgonine hydrochloride	Norcodein	D,L-Propranolol
Ecgonine methylester	Norethindrone	D, L-Tryptophan
(IR,2S)-(-)-Ephedrine	D-Norpropoxyphene	Tyramine
L-Ephedrine	Noscapine	D, L-Tyrosine
(-)-ψ- Ephedrine	D,L-Octopamine	Uric acid
Erythromycin	Oxalic acid	Verapamil
b-Estradiol	Oxazepam	Zomepirac
Cocaethylene	(±) - 3,4-Methylenedioxy-	(±)-Brompheniramine
(±) Isoproterenol	amphetamine	3-Acetate
Morphine Sulfate	(±) - 3,4-Methylenedioxy	D,L-Tyrosine
Tolbutamide	methmphetamine	3-(b-D-glucuronide)
(±) - Chlorpheniramine	Quinacrine	MDE
(-) -ψ-Ephedrine	Dicyclomine	Tryptamine
[1R,2S] (-) Ephedrine	Mehentermine	D,L-Tryptophan
L - Epinephrine	D/L-Propranolol	L-Ψ-Ephedrine
Quindine	Buspirone	Aspirin
Chloroquine	D-Amphetamine	L-Amphetamine
Phenolbarbital	D,L-Amphetamine	Pheniramine
Nimesulide	Creatin	Phenothiazine
b-Hydroxynorephedrtne	Dexbrompheniramine	Phenylethylamine
D/L-Tryptophan	D/L-Octopamine	Quinjdin
Tetrahydrocortisone	Dopamine	Ranitidin
propylamine	Ethanol	Riboflavine
(±) -Brompheniramine	3-(b-D glucuronide)	Glucose
Chloroquin	(+/-)-Isoproterenol	Sodium Chloride
L-Epinephrine	Albumin	Thioridazin
Theophylline	Lidocaine	Trifluoperazin
D/L-Chloropheniramine	Amitriptylin	Trimethobenzamid
Imipramin	3-acetate	Vitamin C
(-)Isoproterenol	D/L-Tyrosine	Benzocaine
(±) -Isoproterenol	Trazodone	Coffeine
Trans-2- phenylcyclopropylamine	p-Hydroxy-methAmphetamine	Guaiacol Glyceryl Ether
Tetrahydrocortisone 3 (b-D glucuronide)	4-Dimethylaminoantipyrine	(+/-)-Chlorpheniramin
(+)3,4-Methylenedioxy-amphetamine	Morphine-3-b-D glucuronide	Morphine-3-b-D-glucuronide
Trans-2-phenylcyclo-propylamine hydrochloride	3,4-Methylenedioxyethyl-Amphetamine	(+) 3,4-Methylenedioxy-methAmphetamine
Tetrahydrocortisone 3 (b-D glucuronide)	Pentazocine hydrochloride	Tetrahydrocortisone, 3 Acetate
Serotonin (5-Hydroxytyramine)	(+) 3,4-Methylenedioxy-amphetamine